QAU Faculty | Microbiology | Dr. Malik Badshah

At Sustaible Bioenerfy and Biorefinery Lab, we seek to provide sustainable solution to country’s need for energy demand to enhance economic growth and development. We are working with plants, fungi, and bacteria in combination with innovative processes to produce next-generation bio-energy sources and sustainable industrial processes. In addition, we are exploring the use of microorganisms for biomass utilization for the production of bio-chemicals and renewable energy sources.

· Exploration of efficient manufacturing methods for bio-based products by exploiting microbial biotechnology to meet this aim (Biorefinery).

· Enhancement of anaerobic digestion of different raw materials (e. g. enzymatic hydrolysis, chemical pretreatment and sonication of industrial and agricultural residues).

· Studying process technology of anaerobic digestion for enhancement and understanding of biogas production.

· Research within the area of production of renewable energy and bio-based chemicals.

· Studying of analytical and computing tools to provide automated tools for process supervision that enables smarter, safer and more cost and time efficient assays of biogas and bioethanol processes for energy production.

· Bio-upgradation of the biogas for utilization as a vehicle fuel.

Peer reviewed Journal papers

1. Noor, H., Satti, S.M., ud Din, S., Farman, M., Hasan, F., Khan, S., Badshah, M. and Shah, A.A., 2020. Insight on esterase from Pseudomonas aeruginosa strain S3 that depolymerize poly (lactic acid)(PLA) at ambient temperature. Polymer Degradation and Stability, p.109096. (IF: 3.780)

2. Rana, Q.U.A., Khan, M.A.N., Irfan, M., Shah, A.A., Hasan, F., Khan, S., Ahmed, S., Adnan, F., Li, W., Ju, M. and Badshah, M.,2019. Starved Spirodela polyrhiza and Saccharomyces cerevisiae: a potent combination for sustainable bioethanol production. Biomass Conversion and Biorefinery, pp.1-10. (IF: 2.326)

3. Haq, A., Siddiqi, M., Batool, S.Z., Islam, A., Khan, A., Khan, D., Khan, S., Khan, H., Shah, A.A., Hasan, F., Ahmed, S. and Badshah, M., 2019. Comprehensive investigation on the synergistic antibacterial activities of Jatropha curcas pressed cake and seed oil in combination with antibiotics. AMB Express, 9(1), p.67. (IF: 2.226)

4. Khan, A., Rahman, U.U., Siddiqui, S., Irfan, M., Shah, A.A., Badshah, M., Hasan, F. and Khan, S., 2019. Preparation and characterization of resistant starch type III from enzymatically hydrolyzed maize flour. Molecular biology reports, 46(4), pp.4565-4580. (IF: 2.107)

5. Khan, S., Ali, I., Badshah, M., Khan, M.Q., Haider, Z., Ali, S., Khan, I.A. and Ullah, A., 2019. Molecular Epidemiology of Hepatitis C Virus Genotypes Among Chronically Infected Patients in Pakistan. Jundishapur Journal of Microbiology, 12(3), pp.1-7. (IF: 0.957)

6. Rana, Q.U.A., Irfan, M., Ahmed, S., Hasan, F., Shah, A.A., Khan, S., Rehman, F.U., Khan, H., Ju, M., Li, W. and Badshah, M.,2019. Bio-catalytic transesterification of mustard oil for biodiesel production. Biofuels, pp.1-8. (IF: 1.130)

7. Ullah, S., Irfan, M., Sajjad, W., Rana, Q.U.A., Hasan, F., Khan, S., Badshah, M. and Ali Shah, A., 2019. Production of an alkali-stable xylanase from Bacillus pumilus K22 and its application in tomato juice clarification. Food Biotechnology, 33(4), pp.353-372. (IF: 0.676)

8. ul ain Rana, Q., Rehman, M.L.U., Irfan, M., Ahmed, S., Hasan, F., Shah, A.A., Khan, S. and Badshah, M., 2019. Lipolytic bacterial strains mediated transesterification of non-edible plant oils for generation of high quality biodiesel. Journal of bioscience and bioengineering, 127(5), pp.609-617. (IF: 2.032)

9. Hameed, S.A., Riffat, R., Li, B., Naz, I., Badshah, M., Ahmed, S. and Ali, N., 2019. Microbial population dynamics in temperature‐phased anaerobic digestion of municipal wastewater sludge. Journal of Chemical Technology & Biotechnology, 94(6), pp.1816-1831. (IF: 2.659)

10. Satti, S.M., Abbasi, A.M., Marsh, T.L., Auras, R., Hasan, F., Badshah, M., Farman, M. and Shah, A.A., 2019. Statistical optimization of lipase production from Sphingobacterium sp. strain S2 and evaluation of enzymatic depolymerization of Poly (lactic acid) at mesophilic temperature. Polymer degradation and stability, 160, pp.1-13. (IF: 3.780)

11. Imtiaz, N., Niazi, M.B.K., Fasim, F., Khan, B.A., Bano, S.A., Shah, G.M., Badshah, M., Menaa, F. and Uzair, B., 2019. Fabrication of an Original Transparent PVA/Gelatin Hydrogel: In Vitro Antimicrobial Activity against Skin Pathogens. International Journal of Polymer Science, 2019. (IF: 1.892)

12. Sajjad, W., Khan, S., Ahmad, M., Rafiq, M., Badshah, M., Zada, S., Khan, S., Hasan, F. and Shah, A.A., 2018. Effects of ultra-violet radiation on cellular proteins and lipids of radioresistant bacteria isolated from desert soil. Folia Biologica, 66(1), pp.41-52. (IF: 1.073)

13. Uzair, B., Kausar, R., Bano, S.A., Fatima, S., Badshah, M., Habiba, U. and Fasim, F., 2018. Isolation and molecular characterization of a model antagonistic Pseudomonas aeruginosa divulging in vitro plant growth promoting characteristics. BioMed research international, 2018. (IF: 2.197)

14. Khan, S., Shinwari, M.I., Haq, A., Ali, K.W., Rana, T., Badshah, M. and Khan, S.A., 2018. Fourier-transform infrared spectroscopy analysis and antifungal activity of methanolic extracts of Medicago parviflora, Solanum nigrum, Melilotus alba and Melilotus indicus on soil-borne phytopathogenic fungi. Pak. J. Bot, 50(4), pp.1591-1598. (IF: 0.672)

15. Hashmi, M., Khan, T.K., Khan, W., Hasan, F., Hameed, A., Khan, S., Badshah, M. and Shah, A.A., 2018. Comparison between a newly isolated yeast strain and Lalvin EC-1118 for enhanced ethanol yield from sugarcane molasses employing batch and modified fed-batch fermentation. Journal of Biobased Materials and Bioenergy, 12(1), pp.134-142. (IF: 2.993)

16. Irfan, M., Tayyab, A., Hasan, F., Khan, S., Badshah, M. and Shah, A.A., 2017. Production and characterization of organic solvent-tolerant cellulase from Bacillus amyloliquefaciens AK9 isolated from hot spring. Applied biochemistry and biotechnology, 182(4), pp.1390-1402. (IF: 2.140)

17. Saboor, A., Khan, S., Ali Shah, A., Hasan, F., Khan, H. and Badshah, M., 2017. Enhancement of biomethane production from cattle manure with codigestion of dilute acid pretreated lignocellulosic biomass. International Journal of Green Energy, 14(7), pp.632-637. (IF: 1.302)

18. Haq, F., Ali, H., Shuaib, M., Badshah, M., Hassan, S.W., Munis, M.F.H. and Chaudhary, H.J., 2016. Recent progress in bioethanol production from lignocellulosic materials: A review. International journal of green energy, 13(14), pp.1413-1441.(IF: 1.302)

19. Sajjad, W., Bhatti, T.M., Hasan, F., Khan, S., Badshah, M., Naseem, A.A. and Shah, A.A., 2016. Characterization of sulfur-oxidizing bacteria isolated from acid mine drainage and black shale samples. Pak J Bot, 48(3), pp.1253-1262. (IF: 0.676)

20. Shah, A.A., Nawaz, A., Kanwal, L., Hasan, F., Khan, S. and Badshah, M., 2015. Degradation of poly (ε-caprolactone) by a thermophilic bacterium Ralstonia sp. strain MRL-TL isolated from hot spring. International Biodeterioration & Biodegradation, 98, pp.35-42. (IF:- 3.824)

21. Moshi, A.P., Crespo, C.F., Badshah, M., Hosea, K.M., Mshandete, A.M. and Mattiasson, B., 2014. High bioethanol titre from Manihot glaziovii through fed-batch simultaneous saccharification and fermentation in Automatic Gas Potential Test System. Bioresource technology, 156, pp.348-356. (IF: 6.669)

22. Moshi, A.P., Crespo, C.F., Badshah, M., Hosea, K.M., Mshandete, A.M., Elisante, E. and Mattiasson, B., 2014. Characterisation and evaluation of a novel feedstock, Manihot glaziovii, Muell. Arg, for production of bioenergy carriers: Bioethanol and biogas. Bioresource technology, 172, pp.58-67. (IF: 6.669)

23. Badshah, M., Parawira, W. and Mattiasson, B., 2012. Anaerobic treatment of methanol condensate from pulp mill compared with anaerobic treatment of methanol using mesophilic UASB reactors. Bioresource technology, 125, pp.318-327. (IF: 6.669)

24. Badshah, M., Lam, D.M., Liu, J. and Mattiasson, B., 2012. Use of an automatic methane potential test system for evaluating the biomethane potential of sugarcane bagasse after different treatments. Bioresource technology, 114, pp.262-269. (IF: 6.669)

25. Crespo, C.F., Badshah, M., Alvarez, M.T. and Mattiasson, B., 2012. Ethanol production by continuous fermentation of d-(+)-cellobiose, d-(+)-xylose and sugarcane bagasse hydrolysate using the thermoanaerobe Caloramator boliviensis. Bioresource technology, 103(1), pp.186-191. (IF: 6.669)

Paper accepted:

1. Chaudry, A., Mahnoor, R.A., Khan, F., Hasan, F., Khan, S., Badshah, M., and Shah, A.A., 2020. Antioxidative and radioprotective properties of glycosylated flavonoid, xanthorhamnin from radio-resistant bacterium Bacillus indicus strain TMC-6. Current Microbiology (Accepted). (IF: 1.595).

Book Chapter

1. Muhammad, A., Badshah. M., (2019). ‘Magnetic Nanoparticles: Eco-Friendly Application in Biofuel Production’, in K. A. Abd-Elsalam et al. (ed.) Magnetic Nanostructures, Nanotechnology in the Life Sciences, Springer Nature

Peer reviewed conference papers

1. Badshah, M., Liu, J., Mattiasson, B., The biomethane potential of chemically defined substrates evaluated using Automatic Methane Potential Test System. Proceedings Abbotabd 2011, 4th International Conference on Environmentally Sustainable Development – ESDev-2011. Abbotabad, Pakistan; 24-26 July 2011.

2. Ilyas, A., Badshah, M., Praagh, M,V., Persson K,M., Evaluation of hydrogen gas production from MSWI bottom ash. Proceedings Venice 2010, Third International Symposium on Energy from Biomass and Waste. Venice, Italy; 8-11 November 2010.

Abstract in conference proceedings

1. Khan, S.S. Badshah, M., 2019, ‘Isolation of Bacillus sp. and Pseudomonas aeruginosa from Anaerobic Digestate and their Application as Biofertilizer’, 1st International Conference on Recent Updates in Biotechnology (ICRUB-2019), Islamabad, Pakistan, 16-18 October. (Oral Presentation)

2. Ali, S., Rana, Q., Badshah, M., 2019, ‘Isolation and Optimization of yeast for second generation bioethanol production from sugarcane bagasse’, 1st International Conference on Recent Updates in Biotechnology (ICRUB-2019). Islamabad, Pakistan, 16-18 October. (Oral Presentation)

3. Riaz, F., Rana, Q., Badshah, M., 2019 ‘Process Designed for Treatment of Agricultural Runoff and Bioethanol Production by Exploiting Spirodela polyrhiza’, 1st International Conference on Recent Updates in Biotechnology (ICRUB-2019). Islamabad, Pakistan, 16-18 October. (Oral Presentation)

4. Muhammad, A., Badshah, M., 2019, ‘Enhancement and Up-gradation of Biogas by Coupling Microbial Electrolysis Cell with the Second Stage of the Anaerobic Digestion’, 1st International Conference on Recent Updates in Biotechnology (ICRUB-2019), Islamabad, Pakistan, 16-18 October. (Oral Presentation)

5. Rana, Q., Badshah, M., 2019, ‘Process optimization for production of Bioethanol from Spirodela polyrhiza using indigenously isolated yeast strain’ ECOMB 2019, Karadeniz Technical University, Tabzon, Turkey, 19-21 September. (Poster presentation)

6. Ali, S., Rana, Q., Badshah, M., 2019, ‘Isolation and optimization of Yeast for second generation Bioethanol production from sugarcane bagasse’, ECOMB 2019, Karadeniz Technical University, Tabzon, Turkey, 19-21 September. (Poster presentation)

7. Muhammad, A., Naeem, A., Badshah, M., 2019, ‘Enhancement and upgradation of biogas by coupling microbial electrolysis cell with the second stage of anaerobic digestion’, ECOMB 2019, Karadeniz Technical University, Tabzon, Turkey, 19-21 September. (Poster presentation)

8. Riaz, F., Rana, Q., Badshah, M., 2019, ‘Utilization of Spirodela polyrhiza for treatment ofagricultural runoff and as feedstock for Bioethanol production’, ECOMB 2019, Karadeniz Technical University, Tabzon, Turkey, 19-21 September. (Poster presentation)

9. Zaffar, N., Khan, A., Haq, A., Badshah. M., 2018, ‘Evaluation of antifungal activity of anaerobic digestate and its effect on growth and yield of maize 2018’, International Conference Applied Biotechnology in Mining, Ukraine, 25-27 April. (Oral Presentation)

10. Shamshad, A., Latif, S., Khan, A., Arif, M., Rana, Q., Badshah, M., 2017, ‘Evaluation of Microalgae for Biofuels Production’, 2nd UMT International Conference on Pure and Applied Sciences (2nd UICPAS-2017), Pakistan, 5-7 October. (Oral Presentation)

11. Latif, S., Shamshad, A., Khan, A., Rana, Q., Arif, M., Badshah, M., 2017, ‘Simultaneous nutrients removal from agricultural runoff and biogas production from microalgae’, 2nd UMT International Conference on Pure and Applied Sciences (2nd UICPAS-2017), Pakistan, 5-7 October. (Oral Presentation)

12. Zaffar, N., Khan, A., Haq, A., Badshah, M., 2017, ‘Evaluation of Antifungal Activity of Anaerobic Digestate and its Effect on Growth and Yield of Maize’, 2nd UMT International Conference on Pure and Applied Sciences (2nd UICPAS-2017), Pakistan, 5-7 October. (Oral Presentation)

13. Shoukat, M., Khan, A., Badshah, M., 2017, ‘Current Status of Anaerobic Digestion Technology in Pakistan’, 2nd UMT International Conference on Pure and Applied Sciences (2nd UICPAS-2017), Pakistan, 5-7 October. (Oral Presentation)

14. Khan, M.A.N., Ullah, I., Khan, A., Badshah, M., 2017, ‘Evaluating the effect of process parameters on biogas yield and process stability during anaerobic co digestion of cattle manure with green grocery waste in two stage reactor’ 2nd UMT International Conference on Pure and Applied Sciences (2nd UICPAS-2017), Pakistan, 5-7 October. (Oral Presentation)

15. Badshah, M., Rana, Q., Rehman, M. L., 2016, ‘Bioremediation of vegetable oil industry wastewater and biodiesel production from taramira oil’, International conference on energy for environmental and economic sustainability (ICEEES-2016), Pakistan, 20-23 October. (Oral Presentation)

16. Siddiqi, M., Haq, A., Batool, S. Z., Badshah, M., 2016, ‘Utilization of Jatropha curcus seedcake as a feedstock for biorefinery’, International conference on energy for environmental and economic sustainability (ICEEES-2016), Pakistan, 20-23 October. (Oral Presentation)

17. Rehman, M. L., Rana, Q., Badshah, M., 2016, ‘Production of biodiesel from Jatropha oil using both enzymatic and alkali catalysis in batch reactor’, International conference on energy for environmental and economic sustainability (ICEEES-2016), Pakistan, 20-23 October. (Oral Presentation)

18. Khan, A., Khan, W., Sohail, M., Badshah, M., 2016, ‘Single and two stage anaerobic co-digestion of cattle manure with fruits vegetable waste’, 4th International Conference on Biological and Computer Sciences (C-BICS 2016), Pakistan, 17 December. (Oral Presentation)

19. Rana, Q., Badshah, M., 2016, ‘Mustard oil as a potential feedstock for biodiesel production: optimization of biodiesel production from mustard oil using Pseudomonas aeruoginosa Qu8 KX 12304 via Plackett-Burman design’, 4th International Conference on Biological and Computer Sciences (C-BICS 2016), Pakistan, 17 December. (Oral Presentation)

20. Khan, A., Sohail, M., Khan, W., Jadoon, M.A., Badshah, M., 2016, ‘Effect of biofilm formation on biogas production in two stage anaerobic digestion process’, 4th International Conference on Biological and Computer Sciences (C-BICS 2016),Pakistan, 17 December. (Oral Presentation)

21. Khan, A., Badshah, M., 2015, ‘Effect of co-digestion with green grocery waste on biogas production from mono-digestion of cattle manure’ 10th Biennial International Conference of Pakistan Society of Microbiology: Exploring microbes for future Endeavours, Pakistan, 25-28 March. (Oral Presentation)

22. Haq, A., Badshah, M., 2015, ‘Enhancement of biogas production from different parts of Jatropha cucrcus Linn Fruit by dilute acid pre-treatment’, 10th Biennial International Conference of Pakistan Society of Microbiology: Exploring microbes for future Endeavours, Pakistan, 25-28 March. (Oral Presentation)

23. Haq, A., Badshah, M., Khan, S., 2015, ‘Comparison of antimicrobial activities of different parts of geographically two different species of Jatropha curcus Linn fruit’, 1st international conference on recent innovations in pharmaceutical sciences (ICRIPS-2015), Pakistan, 3-5 March. (Poster Presentation)

24. Haq, A., Badshah, M., 2014, ‘Evaluation of biomethane potential of the Jatropha curcus fruit’, International conference of plant sciences (ICPS), GCU, Pakistan, 22-24 September. (Poster Presentation)

25. Saif, Y., Saboor, A., Khan, A., Badshah, M., 2014, ‘Enhancement of biogas production from cattle manure by temperature control’, International conference of plant sciences (ICPS), GCU, Pakistan, 22-24 September. (Poster Presentation)

26. Badshah, M., Parawira, W., Mattiasson, B., 2012, ‘Biomethane production from paper and pulp waste fibre sludge’, International conference in wastewater treatment and adsorption: Clean water through Bio- and Nano-technology, Lund, Sweden; 7-9 May. (Oral Presentation)

27. Badshah, M., Nasir, Z., Liu, J., Mattiasson, B., 2012, ‘The biomethane potential of lignocellulosic biomass after different treatments evaluated using an Automatic Methane Potential Test System’, Nordic Biogas Conference, Copenhagen, 23-25 April. (Oral Presentation)

28. Ilyas, A., Badshah, M., Persson K,M., 2012, ‘Reuse of MSWI Bottom Ash as Substrate for Hydrogen Production’, ASH UTILISATION 2012- Ashes in a Sustainable Society, Stockholm, Sweden; 25-27 January. (Poster Presentation)

29. Badshah, M., Parawira, W., Mattiasson, B., 2012, ‘Effect of hydrolysing paper and pulp fibre sludge with enzymes on biogas production in anaerobic batch reactors’, 10th IWA Symposium on Forest Industry Wastewater, Chile, Concepción, 8-11 January. (Oral Presentation)