QAU Faculty | Microbiology | Dr. Javid Iqbal Dasti

Infection Biology:

Dr. J. I. Dasti is an Associate Professor at the Department of Microbiology Quaid-i-Azam University Islamabad. In year 2003, he received a three years fellowship award by German Research Foundation to pursue his PhD on “Signature tagged-transposon mutagenesis of Campylobacter jejuni” at University of Goettingen Germany. He was awarded a PhD in microbiology from University of Goettingen in year 2007 under the supervision of Professor of Medicine, Prof. Dr. Uwe Gross and in the same year, he was awarded a two years Post-Doc Fellowship award by the Washington-State University USA in the group of Prof. Dr. Michael E. Konkel (Not-availed). He continued his research work at the University of Goettingen for two more years on Campylobacter jejuni in the same institute before joining Center for Chronic Immunodeficiency (CCI) University of Freiburg where he worked with Prof. Dr. med. Philipp Henneke on a NIH USA funded project focusing on Interplay of TLR2 and lipoproteins in group B streptococcal sepsis. In year 2010, he successfully received funding from German Research Foundation on Campylobacter jejuni research work established at the University of Goettingen during his fellowship. He was re-invited to work at University of Goettingen in collaboration with Charity-University Medicine Berlin. He joined Department of Microbiology Quaid-i-Azam University Islamabad in year 2011 as an Assistant Professor. He was awarded tenure in December 2018 as an Associate Professor. While working at Quaid-i-Azam University, he did collaborative work, joint publications and student exchange with Honorable Nobel laureate Prof. Dr. Charles M. Rice at the Rockefeller University, Prof. Dr. Jacob George, University of Sydeny, Professor Dr. Betsy Foxman, University of Michigan and Professor Dr Simon Andrews University of Reading, United Kingdom. Most of his research work focuses on infection biology and was funded by German Research Foundation, Commonwealth Split-site Scholarship and International Research Support Initiative Program of Higher Education Commission of Pakistan.

Selected Publications

1. IMTIAZ, W., SYED, Z., RAFAQUE, Z., ANDREWS, S. C. & DASTI, J. I. 2021. Analysis of Antibiotic Resistance and Virulence Traits (Genetic and Phenotypic) in Klebsiella pneumoniae Clinical Isolates from Pakistan: Identification of Significant Levels of Carbapenem and Colistin Resistance. Infect Drug Resist, 14, 227. JCRIF 4.323

2. IMTIAZ, W., DASTI, J. I. & ANDREWS, S. C. 2021. Draft genome sequence of a carbapenemase-producing (NDM-1) and multidrug-resistant, hypervirulent Klebsiella pneumoniae ST11 isolate from Pakistan, with a non-hypermucoviscous phenotype associated with rmpA2 mutation. J Glob Antimicrob Resist, 25, 359-362. JCRIF 3.611

3. RAFAQUE, Z., ABID, N., LIAQAT, N., AFRIDI, P., SIDDIQUE, S., MASOOD, S., KANWAL, S. & DASTI, J. I. 2020. In-vitro Investigation of Antibiotics Efficacy Against Uropathogenic Escherichia coli Biofilms and Antibiotic Induced Biofilm Formation at Sub-Minimum Inhibitory Concentration of Ciprofloxacin. Infect Drug Resist, 13, 2801. JCRIF 4.332

4. JALIL, I., ARSHAD, M., KHAN, S. & DASTI, J. I. 2020. PNPLA3 and TM6SF2, but Not MBOAT7, Are Associated with Steatosis and HBV Viral Persistence in Pakistani Population. Jundishapur J Microbiol, 13. JCRIF 1.499

5. JALIL, I., ARSHAD, M., KHAN, S. & DASTI, J. I. 2020. The STAT4 and not the IFNL3 variant is associated with hepatitis B virus clearance in a population from the Khyber Pakhtunkhwa region of Pakistan. Arab J Gastroenterol, 21, 91-94. JCRIF 1.541

6. ALI, I., RAFAQUE, Z., AHMED, I., TARIQ, F., GRAHAM, S. E., SALZMAN, E., FOXMAN, B. & DASTI, J. I. 2019. Phylogeny, sequence-typing and virulence profile of uropathogenic Escherichia coli (UPEC) strains from Pakistan. BMC Infect Dis, 19, 1-9. JCRIF 3.401

7. ARSHAD, M., JALIL, I., RAZA, A., MALIK, S. & DASTI, J. I. 2019. Novel polymorphism in the promoter region of HLA-DQB1 is a predictor of anti-HCV therapy response. Jundishapur J Microbiol, 12. JCRIF 1.499

8. RAFAQUE, Z., DASTI, J. I. & ANDREWS, S. C. 2018. Draft genome sequence of a multidrug-resistant CTX-M-15 β-lactamase-producing uropathogenic Escherichia coli isolate (ST131-O25b-H30) from Pakistan exhibiting high potential virulence. J Glob Antimicrob Resist, 15, 164-165. JCRIF 3.611

9. ALI, I. & DASTI, J. I. 2018. Chikungunya virus; an emerging arbovirus in Pakistan. J Pak Med Assoc, 68, 252-7. JCRIF 0.929

10. KAUSAR, A., ANWAR, S., SIDDIQUE, N., AHMED, S. & DASTI, J. I. 2018. Prevalence of avian influenza H9N2 virus among wild and domesticated bird species across Pakistan. Pak J Zool, 50, 1347-1354. JCRIF 0.768

11. NAEEM, A. H., MUMTAZ, S., HALEEM, A., QAZI, M. A., MALIK, Z. A., DASTI, J. I. & AHMED, S. 2017. Isolation and molecular characterization of biosurfactant-producing bacterial diversity of Fimkassar oil field, Pakistan. Arab J Sci Eng, 42, 2349-2359. JCRIF 1.71

12. ALI, I., RAFAQUE, Z., AHMED, S., MALIK, S. & DASTI, J. I. 2016. Prevalence of multi-drug resistant uropathogenic Escherichia coli in Potohar region of Pakistan. Asian Pac J Trop Biomed, 6, 60-66. JCRIF 1.545

13. DASTI, J. I. 2016. Zika virus infections: an overview of current scenario. Asian Pac J Trop Med, 9, 621-625. JCRIF 2.285

14. JALIL, I., ARSHAD, M., RAFAQUE, Z., RAZIQ, F., WAZIR, R., MALIK, S. & DASTI, J. I. 2016. Seroprevalence of HDV among non-hospitalized HBsAg positive patients from KPK-region of Pakistan. Asian Pac J Trop Biomed, 6, 609-613. JCRIF 1.545

15. ULLAH, R. W., ZAHUR, A. B., LATIF, A., DASTI, J. I., IRSHAD, H., AFZAL, M., RASHEED, T., MALIK, A. R. & QURESHI, Z.-U.-A. 2016. Detection of peste des petits ruminants viral RNA in fecal samples of goats after an outbreak in Punjab province of Pakistan: A longitudinal study. Biomed Res Int, 2016. JCRIF 3.362

16. ALSUHAIBANI, E., KIZILBASH, N., MALIK, S., DASTI, J., AL BELADI, F. & EL-MORSHEDI, N. 2016. Polymorphisms in promoter regions of IL-6 and IL-10 genes in breast cancer: a case-control study. Genet Mol Res, 15, 56. JCRIF 0.765

17. ALI, I., RAFAQUE, Z., AHMED, S., MALIK, S. & DASTI, J. I. 2016. Prevalence of multi-drug resistant uropathogenic Escherichia coli in Potohar region of Pakistan. Asian Pac J Trop Biomed, 6, 60-66. JCRIF 1.545

18. ULLAH, S., DASTI, J. I. & MALIK, S. 2015. Descriptive epidemiology of hereditary musculoskeletal and limb defects in the isolated population of Chitral, North-West Pakistan. Res J Med Sci, 31, 1047. JCRIF 0.754

19. ULLAH, R. W., ZAHUR, A. B., LATIF, A., IQBAL, J., ZAHRA, D. R. & SAEED-UL-HASSAN, K. 2015. Mild form of peste des petits ruminants virus (PPRV) in Pakistan. Pak J Zool, 47. JCRIF 0.768

20. FAHIM, H., DASTI, J. I., ALI, I., AHMED, S. & NADEEM, M. 2014. Physico-chemical analysis and antimicrobial potential of Apis dorsata, Apis mellifera and Ziziphus jujube honey samples from Pakistan. Asian Pac J Trop Biomed, 4, 633-641. JCRIF 1.545

21. BERESWILL, S., FISCHER, A., PLICKERT, R., HAAG, L.-M., OTTO, B., KüHL, A. A., DASTI, J. I., ZAUTNER, A. E., MUñOZ, M. & LODDENKEMPER, C. 2011. Novel murine infection models provide deep insights into the “menage a trois” of Campylobacter jejuni, microbiota and host innate immunity. PLoS One, 6, e20953. JCRIF 3.788

22. TAREEN, A. M., DASTI, J. I., ZAUTNER, A. E., GROß, U. & LUGERT, R. 2011. Sulphite: cytochrome c oxidoreductase deficiency in Campylobacter jejuni reduces motility, host cell adherence and invasion. Microbiology, 157, 1776-1785. JCRIF 2.871

23. DASTI, J. I., TAREEN, A. M., LUGERT, R., ZAUTNER, A. E. & GROß, U. 2010. Campylobacter jejuni: a brief overview on pathogenicity-associated factors and disease-mediating mechanisms. Int J Med Microbiol, 300, 205-211. JCRIF 4.768

24. TAREEN, A. M., DASTI, J. I., ZAUTNER, A. E., GROß, U. & LUGERT, R. 2010. Campylobacter jejuni proteins Cj0952c and Cj0951c affect chemotactic behaviour towards formic acid and are important for invasion of host cells. Microbiology, 156, 3123-3135. JCRIF 2.871

25. DASTI, J.I., GROß, U., POHL, S., LUGERT, R., WEIG, M. AND SCHMIDT-OTT, R. 2007. Role of the plasmid-encoded tet (O) gene in tetracycline-resistant clinical isolates of Campylobacter jejuni and Campylobacter coli. J Med Microbiol, 56, 833-837. JCRIF 2.836

Conferences:

Candida and Candidiasis today: Where are we, and where to go? (IFOCAN) 2005, Germany.

September 25-28, 2005. Annual Meeting of German Society for Hygiene and Microbiology and the Association for General and Applied Microbiology (VAAM), Germany.

Horizons in Molecular Biology. Decoding Nature: Hierarchy of Interactions. March 17-19, 2005. Goettingen, Germany.

October 4th 2006, Annual Meeting of German Society for Hygiene and Microbiology, Germany.

September, 2-5. 2007, 14th International workshop on Campylobacter, Helicobacter and Related Organisms, Zoonosis and public health, Rotterdam, the Netherlands.

October 30th - November 4 2007, Annual Meeting of German Society for Hygiene and Microbiology and Federation of European Microbiological Societies, Germany.

Annual scientific meeting Indonesian association of clinical microbiology 17-27 March 2008, Indonesia.

25th Symposium in Jena, 2-3, June 2008, Campylobacter infection, Germany (verbal presentation).

Flow-jo-Seminar 23. April, 2009, University of Freiburg, Germany

2010, WHO Symposium Hannover, Germany

March 26- 30, 2012, exploring the hidden potential of Microorganisms, German Academic Exchange Service (DAAD), and Pakistan.

February 22-23, 2013 10th Annual Conference of Infectious Diseases, Pakistan.

1^st international conference on Biotechnology, 21-25^th April 2013 at National Institute of Biotechnology and Genetic Engineering, Faisalabad Pakistan.

Poster and verbal presentation at 20^th annual meeting by Medical Research Society of Pakistan, 25^th May 2013 at Shawkat Khanum Memorial Cancer Hospital Lahore Pakistan.

Arranged a one-day symposium on Blood Donation “Donate to the deserving” at 8 October 2013, at Quaid-i-Azam University Islamabad.

Poster presentation at 11th annual conference on infectious disease, by Medical Microbiology and Infectious Diseases Society of Pakistan, at 14-15^th March 2014 Hotel Margala, Islamabad.

Research data (Abstract submitted w/o participation) at 24th ECCMID (European Congress of Clinical Microbiology and Infectious diseases by ESCMID (European Society of Clinical Microbiology and Infectious Diseases) 10-13 th May 2014 at Barcelona Spain.

From 10-13th May 2014, New Challenges and Frontiers in infectious diseases, Islamabad, Pakistan (Posters & verbal presentations)

From 30 August to 4 September 2014, Session: CSI-02_Inflammation & disease (Poster), FEBS EMBO 2014 Conference, Paris, France

International Conference on Medical Biological and Pharmaceutical Sciences (ICMBPS), Berlin, Germany, November 29, 2015 (verbal presentations).

Poster presentation at first international conference on advancements in Biotechnology 30^th -31^st March 2016 at GC Women University Faisalabad.

International symposium on Nano medicines: Development, Testing, and Application possibilities organized by Nanotheranostics Lab, Nuclear Medicine Oncology and Radiotherapy Institute, Pakistan Atomic Energy Commission Islamabad on 1^st March 2016.

One-day symposium on Burden of Antibiotic Resistance in Neonates Quaid-e-Azam University, Islamabad 29 March 2016

One-day symposium on the theme of Frontiers in Biology: From Discovery to Applications, Allama Iqbal Open University, Islamabad, 9 Jan 2017

Presenting poster of M.Phil. Research work entitled “Efficacy of ceftazidime and Trimethoprim on planktonic cells and biofilms of Uropathogenic E.coli” at 11^th International Biennial Conference of Pakistan Society of Microbiology 18-20 Dec 2018.

Scientific Projects and Funding

· Commonwealth Research Support; 2019-2020; University of Reading, United Kingdom

· Commonwealth Research Support; 2016-2017; University of Reading, United Kingdom

· University Indigenous Research Fund, Year 2012-2018

· URF-QAU 2012-2018

· IPFP, HEC, Pakistan

· Deciphering Molecular Mechanisms of virulence of Campylobacter infection, University of Göttingen, Germany (Funded by DFG).

· To establish in vitro model airways to investigate the role of Toll-like receptors in mucociliary clearance and pneumococcal infection, University of Freiburg, Germany (Funded by DFG).

· To investigate Interplay of TLR2 and lipoproteins in group B streptococcal sepsis, University of Freiburg, Germany (Funded by NIH, USA in collaboration with Pasteur Institute, Paris, France

· Identification and characterization of Campylobacter jejuni factors relevant for the infection process, University of Göttingen, Germany (Funded by DFG, via GRK335).